I have taken inspiration from a Christmas snowflake decoration pattern, and would like to replicate this image with the Opentrons Automation lab system in order to transfer this image onto a black Agar-coated Petri dish plate.
I want to design a snowflake pattern by putting down three different types of fluorescent-protein expressing E. coli strains. E. coli strains are engineered with different high expression plasmids, each encoding for the production of RFP, GFP and m-Orange 2 fluorescent proteins.
In order to render the finl disposition of single colonies and to generate the coordinates accordin to which the single colonies will need to be disposed on the Petri dish, I decided to use the following settings onto the Opentrons Art Interface (https://opentrons-art.rcdonovan.com/ ****):
The Opentrons Colab suite and Python programming language will be used to automate the disposition of bacteria in a three-coloured snowflake pattern by taking advantage of a automated pipettor suite (Opentrons 2 automated robot disposition suite programmed by Python as well as via the Opentrons Colab suite).
Below, I highlight the steps I used to generate the artwork that will be then will be used to extrapolate the coordinates
Three colours will be used
The finished snowflake artwork is as follows:
The following coordinates are generated by the Opentrons Art Interface Program for the disposition of the colonies in space. Coordinates are generated as (x,y) with (0,0) coordinate starting from the center of the Petri Dish plate (0.0).
With reference to the origin (center of the Petri Dish), the following coordinates are generated: